Quantitative Histological Analysis of Ligamentum Flavum
- Mantu Jain, Mukund Namdev Sable, Amit Tirpude
- Received January 09, 2018 Accepted January 09, 2018
The authors used hematoxylin & eosin (HE) and Orcein dyes to look for hemosiderin, amyloid, collagen fibres, elastin, and calcification. HE stain may not be able to differentiate amyloid with elastin and fibres, so did the authors use some special stains like Congo red? This could lead to a higher value of elastin we fear or no amyloid being observed as reported.
The authors have interpreted their results with collagen concentrations in mg/mL. They have not elaborated the methodology how they have measured this with stains. Did they do some morphometric analysis or use some scoring criterion?
In results they have quantified elastic fibres malalignment in numbers which is not described as to how in methodology.
- Notes
- Notes
Conflict of Interest: No potential conflict of interest relevant to this article was reported.
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